Background. One of the important reasons for the emergence of drug-resistant tuberculosis (TB) has been inadequate knowledge and practice of the treatment of TB. The present study was designed to assess the knowledge, attitude and practice of allopathic practitioners of Lucknow with regard to the Revised National Tuberculosis Control Programme (RNTCP) of India. Methods. All allopathic doctors in the city who have experience of more than six months, who see more than five cases of TB per month were interviewed in this questionnaire-based, cross-sectional study. Results. A total of 772 doctors were interviewed. Only 119 (15.4%) claimed to be trained in RNTCP; more than half (n = 452; 58.5%) did not want to take training. Although 88.1% said that clinical examination and sputum testing are required to make the diagnosis, 76.3% doctors said that three sputum samples are required for the diagnosis. Only 43.3% were aware that more than two weeks of cough is a criteria for suspecting TB. Nearly half of the doctors (49.8%) believed that in order to label a patient sputum smear-positive, the chest radiograph should be abnormal. The correct knowledge about categorisation as Category I and Category II was done by 20.4%, and 10.1% respectively; 21.2% still believed that Category III existed. Majority of doctors (92.1%) had a good attitude about RNTCP. Conclusions. Most of the practitioners were aware about RNTCP; however, they did not get training from RNTCP. They should be trained time to time for better services of RNTCP.