Anti-inflammatory and Immunomodulatory Effects of Albizia lebbeck in Experimental Model of Bronchial Asthma
Sulekha Chaudhary, Kavita Gulati, Nishant Rai, Arunabha Ray
Bronchial asthma, Ovalbumin, Inflammation, Immunomodulation and Albizia lebbeck
Citation Information :
Chaudhary S, Gulati K, Rai N, Ray A. Anti-inflammatory and Immunomodulatory Effects of Albizia lebbeck in Experimental Model of Bronchial Asthma. Indian J Chest Dis Allied Sci 2018; 60 (3):147-152.
Background and objective. Bronchial asthma is a heterogeneous disease, usually characterised by chronic airway inflammation, reversible airway narrowing, airway hyperresponsiveness (AHR) and airway remodelling. The study was designed to evaluate the anti-inflammatory and immunomodulatory potentials of Albizia lebbeck in experimental model of bronchial asthma in rats.
Methods: Wistar rats were immunised with ovalbumin (OVA) on day 0 and were challenged with 1% OVA aerosol for 20 minutes daily, from 15th to 22nd day to induce inflammatory model of bronchial asthma. Standardised aqueous extract of Albizia lebbeck (bark) was administered orally for 22 days at doses of 100, 200 and 400 mg/kg, in separate groups. Rats were anesthetised and blood and bronchoalveolar lavage (BAL) fluid were collected and analysed for markers of inflammation (inflammatory cell counts, tumour necrosis factor-alpha [TNF-á], interleukin-6 [IL-6]) and immunomodulation [Ova sIgE], IL-4 and interferon-gamma [IFN-ã]).
Results: Pre-treatment with Albizia lebbeck significantly attenuated the levels of eosinophils, neutrophils, OVA sIgE, TNF-á, IL-6, IL-4 whereas, elevated the levels of IFN-ã in both blood and BAL fluid, thus validating the antiinflammatory and immunomodulatory effect of the extract.
Conclusion: Taken together, the results showed that standardised extract of Albizia lebbeck has anti-inflammatory and immunomodulatory activity as evident from the modulation of cellular and molecular markers of inflammation and immunity in the model of airway inflammation.
Kale RN, Patil RN, Patil RY. Asthma and herbal drugs. Int J Pharm Sci Res 2010;1:37–42.
Ojha NK, Tiwari SK. Ayurveda drugs for management of respiratory allergic disorders: a short review. Int J Innov Res Dev 2014;3:269–75.
Babu NP, Pandikumar P, Ignacimuthu S. Anti-inflammatory activity of Albizia lebbeckBenth., an ethnomedicinal plant, in acute and chronic animal models of inflammation. J Ethnopharmacol 2009;125:356–60.
Abdureyim S, Amat N, Umar A, Upur H, Berke B, Moore N. Anti-inflammatory, immunomodulatory, and heme oxygenase-1 inhibitory activity of RavanNapas: a formulation of uighur traditional medicine in rat model of allergic asthma. Evidence-based Complement Altern Med 2011;10:1155–68.
Wynn TA. Type 2 cytokines: mechanisms and therapeutic strategies. Nat Rev Immunol 2015;15:271–82.
Barnes PJ. Corticosteroid resistance in patients with asthma and chronic obstructive pulmonary disease. J Allergy Clin Immunol 2013;131:636–45.
Verma SC, Vashishth E, Singh R, Kumari A, Meena AK, Pant P, et al. A review on parts of Albizia lebbeck (L.) benth: used as ayurvedic drugs. Res J Pharm Technol 2013;6:1307–13.
Chaudhary S, Gulati K, Rai N, Ray A. Effects of aqueous extract of Solanumxanthocarpum in experimental model of bronchial asthma and its mechanisms. EC Pharmacol Toxicol 2016;2:241–50.
Rai N, Ray A, Jamil SS, Gulati K. Cellular and molecular mechanisms of action of UNIM-352, a polyherbal preparation, in experimental models of bronchial asthma. Indian J Exp Biol 2015;53:625–31.
Horn BR, Robin ED, Theodore J, Van Kessel A. Total eosinophil counts in the management of bronchial asthma. N Engl J Med 1975;292:1152–5.
Wenzel SE, Szefler SJ, Leung DY, Sloan SI, Rex MD, Martin RJ. Bronchoscopic evaluation of severe asthma: persistent inflammation associated with high dose glucocorticoids. Am J Respir Crit Care Med 1997;156:737–43.
Kuhl K, Hanania NA. Targeting IgE in asthma. Curr Opin Pulm Med 2012;18:1–5.
Rincon M, Irvin CG. Role of IL-6 in asthma and other inflammatory pulmonary diseases. Int J Biol Sci 2012;8:1281.
Shah A, Church MK, Holgate ST. Tumour necrosis factor alpha: a potential mediator of asthma. Clin Exp Allergy 1995;25:1038–44.
Hsieh CS, Heimberger AB, Gold JS, O'Garra A, Murphy KM. Differential regulation of T helper phenotype development by interleukins 4 and 10 in an alpha beta T-cell-receptor transgenic system. Proc Natl Acad Sci 1992;89:6065–9.
Leonard C, Tormey V, Burke C, Poulter LW. Allergeninduced cytokine production in atopic disease and its relationship to disease severity. Am J Respir Cell Mol Biol 1997;17:368–75.