Background: With an increasing prevalence rate of tobacco use in India in the last few years, Ministry of Health and Family Welfare, Government of India launched the first ever “National Tobacco Quitline Services (NTQLS)” on 30th May, 2016 at Vallabhbhai Patel Chest Institute, University of Delhi (North Campus), Delhi. This is a telephonebased tobacco cessation service, an important component of many tobacco control programmes. Methods: NTQLS is established to help a person who wants to quit his/her habit of tobacco use. Services at NTQLS are available daily from 8 AM to 8 PM through a National toll-free number 1800-11-2356 in both English and Hindi, except on Monday. Six counsellors were present at a time both during morning and evening shift. A total of 5179 callers were registered from 30th May 2016 to 31st May 2017. The comparative analysis was done between the smokers (n=1366) and smokeless tobacco users (n=3169) those registered at NTQLS using statistical package for the social sciences (SPSS, version 22). Tobacco smokers who used smokeless tobacco (n=644) were excluded from the present study. Results: A total of 1366 smokers and 3169 smokeless tobacco users were studied. Tobacco consumption was higher in males as compared to females in both the groups. Individuals in the age group of 25 to 64 years were found to be more prone to smokeless tobacco consumption as compared to smoking tobacco (69.5% versus 67.6%). Out of 1366 registered callers who smoked tobacco, the success rate of quitters was 33.9% and out of 3169 smokeless tobacco users, the success rate of quitters was 41% during the study period. Conclusions: Results of this telephone-based counselling services at NTQLS indicate this to be helpful in motivating people to quit or stop the use of tobacco. Overall findings revealed a significant reduction in number of tobacco smokers as compared to users of smokeless tobacco at the end of one year of study period after availing NTQLS services for quitting.