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Evaluating a Modified Ziehl-Neelsen Technique with Triton X-100 for Detecting Acid-fast Bacilli in Sputum

Anisha Garg, Biswaroop Chatterjee, Aarti Kotwal, Rishabh Bansal, Indranil Saha

Citation Information : Garg A, Chatterjee B, Kotwal A, Bansal R, Saha I. Evaluating a Modified Ziehl-Neelsen Technique with Triton X-100 for Detecting Acid-fast Bacilli in Sputum. Indian J Chest Dis Allied Sci 2022; 64 (2):124-125.

DOI: 10.5005/jp-journals-11007-0003

License: CC BY-NC 4.0

Published Online: 08-07-2022

Copyright Statement:  Copyright © 2022; The Author(s).


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  1. World Health Organization (WHO) [homepage on the Internet]. Geneva: Global Tuberculosis report 2020. Available from: https://apps.who.int/iris/bitstream/handle/10665/336069/9789240013131-eng.pdf [Accessed on December 22, 2020].
  2. Parsons LM, Somoskövi A, Gutierrez C, et al. Laboratory diagnosis of tuberculosis in resource-poor countries: challenges and opportunities. Clin Microbiol Rev 2011;24(2):314–350. DOI: 10.1128/CMR.00059-10.
  3. Yeager H Jr, Lacy J, Smith LR, et al. Quantitative studies of mycobacterial populations in sputum and saliva. Am Rev Respir Dis 1967;95(6):998–1004. DOI: 10.1164/arrd.1967.95.6.998.
  4. Singh NP, Parija SC. The value of fluorescence microscopy of auramine stained sputum smears for the diagnosis of pulmonary tuberculosis. Indian J Path Microbiol 1998;29(4):860–863. PMID: 10772577.
  5. Koch ML, Cote RA. Comparison of fluorescence microscopy with Ziehl-Neelsen stain for demonstration of acid-fast bacilli in smear preparations and tissue sections. Am Rev Respir Dis 1965;91:283–284. DOI: 10.1164/arrd.1965.91.2.283.
  6. Chen P, Shi M, Feng GD, et al. A highly efficient Ziehl-Neelsen stain: identifying de novo intracellular Mycobacterium tuberculosis and improving detection of extracellular M. tuberculosis in cerebrospinal fluid. J Clin Microbiol 2012;50(4):1166–1170. DOI: 10.1128/JCM.05756-11.
  7. Ministry of Health and Family Welfare [homepage on the Internet]. Delhi: Module for Laboratory Technicians. Available from: https://tbcindia.gov.in/WriteReadData/l892s/6341531217Module%20for%20Laboratory%20Technician.pdf [Accessed on October 13, 2016].
  8. Ministry of Health and Family Welfare [homepage on the Internet]. Delhi: Manual for Sputum Smear Fluorescent Microscopy. Available from: http://tbcindia.nic.in/WriteReadData/l892s/7890638455Flourescence_Microscopy%20Manual.pdf [Accessed on October 13, 2016].
  9. Feng GD, Shi M, Ma L, et al. Diagnostic accuracy of intracellular mycobacterium tuberculosis detection for tuberculous meningitis. Am J Respir Crit Care Med 2014;189(4):475–481. DOI: 10.1164/rccm.201309-1686OC.
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